PoplarV1, Main, Exploration, bibRecord, 003671

Cytogenetic analysis of Populus trichocarpa--ribosomal DNA, telomere repeat sequence, and marker-selected BACs.

Identifieur interne : 003671 ( Main/Exploration ); précédent : 003670; suivant : 003672

Cytogenetic analysis of Populus trichocarpa--ribosomal DNA, telomere repeat sequence, and marker-selected BACs.

Auteurs : M N Islam-Faridi [États-Unis] ; C D Nelson ; S P Difazio ; L E Gunter ; G A Tuskan

Source :

Cytogenetic and genome research [ 1424-859X ] ; 2009.

RBID : pubmed:19617699

Descripteurs français

English descriptors

KwdEn :
- Arabidopsis (genetics), Chromosomes, Artificial, Bacterial (genetics), Chromosomes, Plant (genetics), Cytogenetic Analysis (MeSH), DNA, Plant (genetics), DNA, Ribosomal (genetics), Genetic Markers (MeSH), In Situ Hybridization, Fluorescence (MeSH), Populus (classification), Populus (genetics), Repetitive Sequences, Nucleic Acid (MeSH), Species Specificity (MeSH), Telomere (genetics).
MESH :
- chemical , genetics : DNA, Plant, DNA, Ribosomal.
- classification : Populus.
- genetics : Arabidopsis, Chromosomes, Artificial, Bacterial, Chromosomes, Plant, Populus, Telomere.
- Cytogenetic Analysis, Genetic Markers, In Situ Hybridization, Fluorescence, Repetitive Sequences, Nucleic Acid, Species Specificity.

Abstract

The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.

DOI: 10.1159/000218749
PubMed: 19617699

Affiliations:

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Le document en format XML

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<term>Cytogenetic Analysis (MeSH)</term>
<term>DNA, Plant (genetics)</term>
<term>DNA, Ribosomal (genetics)</term>
<term>Genetic Markers (MeSH)</term>
<term>In Situ Hybridization, Fluorescence (MeSH)</term>
<term>Populus (classification)</term>
<term>Populus (genetics)</term>
<term>Repetitive Sequences, Nucleic Acid (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term>
<term>ADN ribosomique (génétique)</term>
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<term>Chromosomes artificiels de bactérie (génétique)</term>
<term>Chromosomes de plante (génétique)</term>
<term>Hybridation fluorescente in situ (MeSH)</term>
<term>Marqueurs génétiques (MeSH)</term>
<term>Populus (classification)</term>
<term>Populus (génétique)</term>
<term>Spécificité d'espèce (MeSH)</term>
<term>Séquences répétées d'acides nucléiques (MeSH)</term>
<term>Télomère (génétique)</term>
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<term>Genetic Markers</term>
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<term>Séquences répétées d'acides nucléiques</term>
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<front><div type="abstract" xml:lang="en">The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.</div>
</front>
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<CopyrightInformation>Copyright 2009 S. Karger AG, Basel.</CopyrightInformation>
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Cytogenetic analysis of Populus trichocarpa--ribosomal DNA, telomere repeat sequence, and marker-selected BACs.

Cytogenetic analysis of Populus trichocarpa--ribosomal DNA, telomere repeat sequence, and marker-selected BACs.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki